Abstract
A new way of solubilizing hydrolytic enzymes in organic media was achieved by coating the enzyme with lipid monolayers. Lipid-coated enzymes have been used successfully for a number of types of reactions in organic media or organic-aqueous two-phases, for example enantioselective esterification by lipase, transphosphatidylation of water-insoluble phospholipids by phospholipase D, hydrolysis of a lipophilic substrate by a catalytic antibody, and transglycosylation by, β-D-galactosidase.
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