Abstract
A new way of solubilizing hydrolytic enzymes so that they can be used in organic solvents is to coat them with lipid monolayers. Lipid-coated enzymes have been used successfully for a number of different types of reactions in organic media or organic-aqueous, two-phase systems; for example, enantioselective esterification by lipase, transphosphatidylation of water-insoluble phospholipids by phospholipase D, hydrolysis of a lipophilic substrate by a catalytic antibody and transglycosylation by β-d-galactosidase.
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