Abstract
Despite recent advances in biomimetic scaffolds, there is an unmet need to understand a regulatory role of extracellular matrix (ECM) structure in maintaining healthy cardiomyocyte (CM) phenotype. In this study, we investigated how key structural components of electrospun scaffolds regulate CM phenotype. We found that healthy cardiac ECM-mimetic poly(ε-caprolactone) (PCL) nanofiber scaffolds promoted mature CM phenotype with organized actin/myomesin bands in both neonatal and adult CMs with expression of β-MYH7 and SCN5A.1 and SCN5A.2 compared to the failing heart ECM-mimetic scaffold and tissue culture plates This study outcome provides new insight into cardiac tissue engineering by suggesting a tunable model that mimics the complex cardiac microenvironment in vitro, thereby advancing CM biology.
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