Camphortaimide B 抗癌活性及其作用機制之研究

Abstract

Abstract Antrodia camphorata, known as“niu-chang-chin”in Taiwan, is a fungus of the family polyporaceae that grows slowly and rare species. Traditionally, the fruit body of Antrodia camphorata used to treat diarrhea, abdominal pain, hypertension, and liver cancer. Many previous studies reported that the extract of fruit body and mycelium of Antrodia camphorata possesses a wide range of biological functions, such as antioxibant, anti-inflammation, anticancer. However, the relationship of structure bioactive component of Antrodia camphorata and bioactivity is not well known. In this studies, we investigated the anticancer potential of synthetic camphora- taimide B, the maleic derivatives from the mycelium, on MDA-MB-231 breast cancer cells. First, we studied whether camphorataimide B can inhibit breast cancer cells growth by cytotoxicity assay and colony formation assay. The results showed that camphorataimide B can restrain the proliferation of breast cancer cells. In addition, by DAPI staining and DNA fragmentation assay, the results found camphorataimide B induced apoptosis of MDA-MB-231 B breast cancer cells at concentrations of 10-40 μM. Furthermore, camphorataimide B was able to induce cell cycle arrest in G2/M phase. Then, we performed cell scattering, F-actin fluoresence staining, wound healing, and Boyden chamber migration assay to examine anti-migration of camphora -taimide B on breast cancer cells. Moreover, we want to know whether camphorataimi -de B will also inhibit breast cancer cell invasion. We conducted adhesion assay and Boyden chamber invasion assay. We found that camphorataimide B also inhibited breast cancer cells invasion. By weatern blotting analysis, it showed camphora -taimide B suppressed the expression of cathepsin D and HIF-1α, a transcription factor of cathepsin D. Finally, we used animal models to study camphorataimide B on lung colonization of MDA-MB-231 cells in nude mice. The results showed camphora- taimide B reduced the size and weigth of lung as well as lung colonization of MDA-MB-231 cells.