Abstract
A bulk acoustic wave (BAW) impedance sensor has been applied to rapid determination of in vitro concentrations of cefotaxime sodium. The method based on the growth frequency curve of Proteus Morganii, inhibited by cefotaxime sodium, is continuously monitored by using a BAW impedance sensor. The growth kinetic parameter, e.g. asymptote or maximum frequency shift (AF, Hz), estimated by fitting a modified Gompertz model to the growth frequency curve, is proportional to the logarithm of antibiotic concentration. The proposed method exhibits a favourable response to 0.25 – 20.0 μg mL−1 cefotaxime sodium with a detection limit of 0.03 μg mL−1, and the recoveries range from 97.3 to 107.5% (n=9). The results are in good agreement with those obtained by the conventional disk diffusion test (DDT) method. The effects of temperature and pH on the determination are discussed and in vitro susceptibility to cefotaxime of the genus Proteus has been investigated in detail. On leave from Department of Mathematics, Physics and Mechanics, Changsha Railway University.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
