Abstract
The regulatory property of NAD+-isocitrate dehydrogenase (NADMCDH) was studied by means of a new device, a surface acoustic wave (SAW) impedance sensor, which comprises a 61 MHz SAW resonator and a pair of parallel platinum electrodes. The NAD+-ICDH is an allosterically regulated enzyme which shows sigmoidal kinetic for DL-isocitrate. The Hill coefficient was estimated to be 2.93. A linear relationship between the frequency response and the enzyme concentration was obtained, and the experimental detection limit was evaluated to be 45 mlU/ml. A comparison between the SAW impedance sensor and the conductometric method was carried out. The effects of AMP, ADP and ATP on the kinetic property of NAD+-ICDH were investigated; some other factors, such as the temperature and pH value, are also discussed.
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