Abstract
Molecular biology and genetic studies requires high quality DNA isolated from plants. Therefore, isolation of pure and high quality DNA in a short time is important. However, since different plants have different chemical composition, in even close species need specific genomic DNA isolation protocol. There are a large number of protocols for wheat. Therefore, which method is more applicable, safer and easier should be determined. For this purpose three DNA extraction protocols were used. The DNA samples extracted from these protocols were compared for their purity and concentrations. The quality and amount of DNA obtained was determined by NanoDrop Spectrophotometer (A260/280 absorbance) and agarose gel electrophoresis. Additionally DNAs extracted were amplified by polymerase chain reactions (PCR) and the suitability of isolated DNAs for PCR reactions were also determined. The results show that with the CTAB DNA extraction methods were extracted the highest DNA concentrations in wheat ( Triticum avestivum L. ) compared to other protocols.
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