BİTKİSEL KÖKENLİ GIDALARDA YÜKSEK POLARİTELİ PESTİSİTLERİN SIVI KROMATOGRAFİ/KÜTLE SPEKTROMETRİSİ YÖNTEMİYLE ANALİZİ

Abstract

Pesticides in foods of plant origin are becoming a major issue due to their intensive use in agriculture. Since pesticides are potentially harmful to the environment and consequently to human health through the consumption of pesticide contaminated foods. The European Community and also the Ministry of Agriculture and Forestry in Turkish Republic established maximum residue levels (MRLs), based on the assumption that good agricultural practice is applied at the use of pesticides in farming, for pesticide residues in food of plant origin. As a consequence food commodities have to be controlled to assure the non-violation of the MRLs. Thus an appropriate control of their residues in samples has to be operated. 
 In this study, we inferred the analytical methods for highly polar pesticides in foods of plant origin via LC-MS/MS after extraction with acidified methanol according to QuPPe (Quick Polar Pesticides) method. Optimization of 13 highly polar pesticides was carried out with LC-MS/MS Q-Trap. After the optimization process has been completed, the samples to be taken according to the SANTE (Guidance document on analytical quality control and method validation procedures for pesticide residues and analysis in food and feed) guidelines have been carefully selected, including high water, high acid/high water, high sugar/low water, high oil/low water and high starch/low water content and difficult or unique commodities. Six samples carefully selected according to the SANTE guideline were carried out at two different concentrations, one of at the detection limit which is 10 µg/L. At the end of the studies, it was found that the RSD criteria for repeatabilitiy was below 20 % for all substances. In addition, the recovery value for all substances was in the range of 70 – 120 %. As a result, 13 different polar pesticides can be analyzed in product groups of plant origin via LC-MS/MS method developed by using hypercarb column.