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Abstract
The supercoiled DNA form of the colicinogenic factor E1, labeled with tritiated thymidine, was stored at −25°C and aliquots examined periodically for breaks in the DNA. The majority of the breaks were single-stranded and took place in the labeled strand of the DNA. Double-stranded breaks due to the tritium disintegration occurred at about one-sixth the efficiency for single-stranded breaks. The formation of alkaline-sensitive bonds as a result of the tritium decay was not observed.
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