Bone Marrow Mesenchymal Stem Co-Culture Inhibited the Proliferation of Endometrial Cancer Cells by Upregulating miR-141-3p/Death Associated Protein Kinase 1 (DAPK1) Axis

Abstract

MicroRNAs are involved in the biological processes of a variety of cancers. Here, we intend to assess the effect of BMSC-derived exosomal miR-141-3p on endometrial cancer (EC) cells. EC tumor and normal tissues were collected to measure miR-141-3p and death associated protein kinase 1 (DAPK1) expression. EC cells transfected with miR-141-3p mimic were cultured with BMSCs followed by measuring cell proliferation, apoptosis and migration. miR-141-3p and DAPK1 level was significantly decreased in EC tissues and cell lines. After miR-141-3p mimics transfection, miR-141-3p and DAPK1 expression was increased significantly, along with inhibited cell proliferation, migration and invasion and increased cell apoptosis. Moreover, co-culture with BMSC decreased EC cells in vitro activity, and upregulated miR-141-3p and DAPK1 expression. In conclusion, miR-141-3p and DAPK1 is reduced in EC and miR-141-3p overexpression inhibited EC cell biological behaviors, indicating that miR-141-3p might be a potential target for treatment of EC.