Abstract
Previous studies revealed that blue light stimulates and red light inhibits prehaustoria development in young seedlings of the parasitic weed Cuscuta campestris (field dodder). This study showed a positive correlation between blue light induced increases in inositol 1,4,5-trisphosphate (IP3) and blue light-mediation of prehaustoria development, prior to host attachment. Blue light induced a significant increase in the level of IP3, with a peak at about 30 min. Thereafter, the level of IP3 declined to the resting value after 2 h of blue light. Irradiation with 10 min red light pulse applied directly at the end of each 0.05, 0.1, 0.15, 0.2, 0.3, 1, 2 and 4 h blue light significantly reduced IP3, while high levels of IP3 were observed after 10 min far-red pulse. The G-protein inhibitor pertussis toxin inhibited prehaustoria developed under blue light, suggesting that receptor-coupled G-proteins are likely to be involved in prehaustoria development. These results are the first in vivo demonstration of a possible role for IP3 as a second messenger in the blue light signal transduction process in prehaustoria development in Cuscuta.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call