Blood vessel count and bone formation after maxillary sinus augmentation

Abstract

Event Abstract Back to Event Blood vessel count and bone formation after maxillary sinus augmentation Christoph Brochhausen1, 2, Volker H. Schmitt1, Sebastian Kuehl3, Christine Schmitt1, Andreas Mamilos1, 2, Andreas Filippi3, Matthias Kreisler3 and C James Kirkpatrick1 1 University Medical Centre Mainz, REPAIR-lab, Institute of Pathology, Germany 2 University Hospital Regensburg, REPAIR-lab, Institute of Pathology, Germany 3 Univeristy Basel, Clinic for Oral Surgery, Switzerland Introduction: Maxillary sinus augmentation (MSA) represents a valuable technique to successfully graft severe atrophic maxillary bone prior to implant placement. For this purpose, autologous bone, bone substitute materials or mixtures of both can be used. The aim is to obtain a maximum amount of bone. Angiogenesis is a crucial step in bone formation. However, there are no studies which have evaluated the correlation between blood vessel count and the amount of bone after MSA. In this study, the correlation between the number of blood vessels and the percentage distribution of bone as well as soft tissue was investigated after MSA with bone and bone substitute materials in ex vivo samples. Materials and Methods: 35 trephine samples were retrieved five months after grafting the maxillary sinus either with autologous bone (control, n=8), composites of autologous bone and beta-tricalcium phosphate (n=15, group A) or autologous bone and beta-tricalcium phosphate/hydroxyapatite (n=11, group B). The samples were processed according to standardized methods for histological (HE, Goldner) and immunohistochemical (anti-CD31 stain) examination. The vessel count per sample was determined as well as the distribution of bone, substitute biomaterial and soft tissue. The data were plotted against the percentage distribution of bone and soft tissue and correlations were evaluated by means of a Spearman test. Results: The mean distribution of soft tissue was the highest in group A (42.3 %) and lowest in group B (32.2 %). The mean distribution of bone was the highest in the control (27.8 %), followed by group A (mean=24.2%). The lowest amount of bone was seen in group B (mean=23.5 %). The control group gave the highest number of blood vessels (mean=53.8 vessels per sample). Group A revealed 47.1 vessels per sample, while the lowest number (26.9 vessels per sample) was detected in group B. Discussion: In this study, blood vessel count correlated significantly with the amount of soft tissue. No correlation could be found between the number of blood vessels and the percentage distribution of bone. In addition, angiogenesis occurred mainly in soft tissue independent of the grafted material used after MSA. Further studies are necessary to investigate the mechanisms of bone formation and the role of the blood supply in MSA. Keywords: Bone Regeneration, Biomimetic Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Bone substitutes in clinics Citation: Brochhausen C, Schmitt VH, Kuehl S, Schmitt C, Mamilos A, Filippi A, Kreisler M and Kirkpatrick C (2016). Blood vessel count and bone formation after maxillary sinus augmentation. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02955 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Christoph Brochhausen Volker H Schmitt Sebastian Kuehl Christine Schmitt Andreas Mamilos Andreas Filippi Matthias Kreisler C James Kirkpatrick Google Christoph Brochhausen Volker H Schmitt Sebastian Kuehl Christine Schmitt Andreas Mamilos Andreas Filippi Matthias Kreisler C James Kirkpatrick Google Scholar Christoph Brochhausen Volker H Schmitt Sebastian Kuehl Christine Schmitt Andreas Mamilos Andreas Filippi Matthias Kreisler C James Kirkpatrick PubMed Christoph Brochhausen Volker H Schmitt Sebastian Kuehl Christine Schmitt Andreas Mamilos Andreas Filippi Matthias Kreisler C James Kirkpatrick Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.