Abstract
Rubus idaeus L. is one of the oldest and most widespread berry crops, which is cultivated for the excellent taste as well as for medical and dietary properties of berries. Rubus fruits contain a significant amount of vitamins A and C, anthocyanins, polyphenolic substances, which determines their high antioxidant activity. Remontant red raspberry forms are able to berry on annual shoots in the second half of summer, which extends the term of consumption of fresh berries by 1.5-2 months. However, many forms of remontant raspberry have a low potential for vegetative propagation compared to summer varieties, which makes them difficult to reproduce and to use in the breeding process. We investigated the possibility of increasing the efficiency of in vitro micropropagation of a remontant raspberry variety ʻBiryulevskaya’. The effects of 6-benzylaminopurine (6-BAP) at concentrations of 0.5–3 mg l-1 and thidiazuron (TDZ) at concentrations of 0.05–0.2 mg l-1 as well as doubled and tripled iron chelate Fe-EDTA (Ferric ethylenediamine-tetraacetic acid) doses were studied. We found the adding 1.0 mg l-1 6-BAP to the MS medium containing a triple dose of iron chelate, provided intensive proliferation of high quality adventitious shoots.
Highlights
The raspberry (Rubus idaeus L.) belongs to the Rosaceae family
It became necessary to optimize the process of clonal micropropagation of remontant forms of raspberries
We found that 6-BAP and TDZ were the most effective growth regulators of the cytokinin series
Summary
The raspberry (Rubus idaeus L.) belongs to the Rosaceae family. It is one of the oldest and most widespread berry crops, which is cultivated for the excellent taste as well as for medical and dietary properties of berries. They serve as valuable raw materials for the food and confectionery industry. Numerous studies have been conducted in different countries to improve the method of clonal micropropagation in order to produce high-quality raspberry planting material. The biological features of the remontant forms of raspberries, associated with their complex interspecific origin, have led to the low effectiveness of the proposed biotechnological methods for the reproduction of raspberries at some stages of in vitro cultivation. In this regard, it became necessary to optimize the process of clonal micropropagation of remontant forms of raspberries. The aim of this work was to study in vitro growth and development of red raspberry microshoots at the stage of micropropagation
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