Abstract
Sulphur-35 from labelled sulphate, sulphide, thiosulphate and methionine was incorporated into sinigrin isolated from mature horse-radish leaves. Sulphate-S was more readily incorporated than was that from sulphide, thiosulphate or methionine. When inorganic sulphur-35 was supplied, about 80 per cent of the radioactivity of sinigrin was in the bisulphate moiety and 20 per cent in the isothiocyanate. However, with methionine- 35S as the tracer the isothiocyanate portion contained 90 per cent of the radioactivity. The data suggest that methionine sulphur is incorporated into sinigrin by a different pathway than are the various forms of inorganic sulphur.
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