Abstract
In this study bacterial strainB4A isolated from petroleum hydrocarbon contaminated soil showed potential to produce bio-surfactant in the presence of 2% (v/v) diesel oil as a carbon source. The growth rate was determined using spectrophotometer at 600nm. Identification based on 16S r-RNA sequencing proved B4A isolate as Pantoeadispersa. Bio surfactant production by this strain was found to be growth-linked. Maximum emulsifying activity and Fourier-transform infrared spectroscopic analysis revealed the glycoprotein nature of the bio-surfactant. Results indicated that the isolated strain had effectively utilized crude oil as sole carbon source. Linear increase in optical density was observed between 1-12 days. Pantoeadispersa B4A showed the highest growth of 81% in medium with diesel oil. This study held in isolation of potential hydrocarbon degrading bacteria from contaminated soil and it can further be used for bioremediation of oil contaminated soil. The strain showed production of bio-surfactant which will assist in further effective remediation of oil polluted sites.
Published Version
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