Abstract
A fundamental study of the application of bacteria to the recovery of toxic heavy metals from aqueous environments was carried out. The biosorption characteristics of cadmium and lead ions were determined with purple nonsulfur bacteria,Rhodobacter sphaeroidesand hydrogen bacteria,Alcaligenes eutrophusH16 that were inactivated by steam sterilization. A simplified version of the metal binding model proposed by Pletteet al.was used for the description of metal binding data. The results showed that the biosorption of bivalent metal ions to whole cell bodies of the bacteria was due to monodentate binding to two different types of acidic sites: carboxilic and phosphatic-type sites. The number of metal binding sites ofA. eutrophuswas 2.4-fold larger than that ofR. sphaeroides.
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