Abstract
BackgroundOsteosarcoma (OS) is a type of bone cancer that occurs in children and adolescents at a rate of 5%. The purpose of this study is to explore the lncRNA GNAS-AS1 expression profile, prognosis significance in OS, and biological effect on OS cell function.MethodsOne hundred eight pairs of tissues were collected, and OS cell lines were purchased. lncRNA GNAS-AS1 expression in these tissues and cells were analyzed by qRT-PCR. Clinical data were analyzed using chi-square tests, Kaplan-Meier curves (log-rank test), and Cox regression. CCK-8 and transwell assay were conducted to analyze the effect of lncRNA GNAS-AS1 on cell proliferation, invasion, and migration. The downstream miRNA was presumed.ResultsThe expression of lncRNA GNAS-AS1 was significantly increased in OS cells and tissues, and related to Enneking staging and distant metastasis. Patients with high lncRNA GNAS-AS1 expression represented shorter overall survival and was an independent prognostic predictor of OS. LncRNA GNAS-AS1 knockdown inhibited cell proliferation, migration, and invasion by regulated miR-490-3p partly at least.ConclusionsLncRNA GNAS-AS1 can be used as a prognostic indicator and its inhibition suppress the development of OS, suggesting its value as novel therapeutic strategies in OS.
Highlights
Characterized by mineralizing of osteoid with multinucleated cells, osteosarcoma (OS) is the most common tumor in bone tissues among children and adolescents [1, 2]
Upregulation of long non-coding RNAs (lncRNAs) GNAS-AS1 in OS tissues and cell lines To obtain the expression profile of lncRNA GNAS-AS1 in OS, quantitative real-time PCR (qRT-PCR) was utilized to determine its level in OS tissues, the matched normal tissues, OS cell lines, and normal osteoblast cell line
Compared to the normal tissues, lncRNA GNAS-AS1 was found with an ascendant expression level in OS tissues (P < 0.001, Fig. 1A)
Summary
Characterized by mineralizing of osteoid with multinucleated cells, osteosarcoma (OS) is the most common tumor in bone tissues among children and adolescents [1, 2]. Mi et al Journal of Orthopaedic Surgery and Research (2021) 16:470 cancers recently, since they are usually aberrantly expressed in disease tissues and promote or inhibit tumor development, progression, and metastasis [8,9,10,11]. LncRNA GNAS-AS1 was reported to facilitate nasopharyngeal carcinoma, ER+ breast cancer, and lung cancer cell progression [17,18,19]. Osteosarcoma (OS) is a type of bone cancer that occurs in children and adolescents at a rate of 5%. The purpose of this study is to explore the lncRNA GNAS-AS1 expression profile, prognosis significance in OS, and biological effect on OS cell function
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