LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion.

Biyong Deng,Taizhe Wang,Weiguo Wang,Runsang Pan,Yingjie Nie,Houping Chen,Xin Ou,K H Mok

doi:10.1155/2021/5271291

Biyong Deng, Taizhe Wang + Show 6 more

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https://doi.org/10.1155/2021/5271291

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Abstract

Purpose Osteosarcoma (Os) is the most frequent malignant tumor of the bone in the pediatric age group, and accumulating evidences show that lncRNAs play a key role in the development of Os. Thus, we investigated the role of RBM5-AS1 and its molecular mechanism. Methods The expression of RBM5-AS1 in Os tissues and cell lines was detected by real-time polymerase chain reaction (QPCR). The effect of RBM5-AS1 on the proliferation of Os cells was detected using CCK8 assays and flow cytometry. The effect of RBM5-AS1 on the migration and invasion of Os cells was detected by transwell assays. And we performed QPCR and western blotting assays to investigate the relationship between RBM5-AS1 and RBM5. Finally, western blotting assays were performed to explore the mechanism of RBM5. Results LncRNA RBM5-AS1 was overexpressed in the Os tissues and cell lines. And lncRNA RBM5-AS1 promoted Os cell proliferation, migration, and invasion in vitro and tumor growth in vivo. LncRNA RBM5-AS1 targets RBM5 in Os cells. Conclusion To sum up, the results showed that lncRNA RBM5-AS1 promotes cell proliferation, migration, and invasion in Os.

Highlights

Osteosarcoma (Os) is the most frequent malignant tumor of the bone in the pediatric age group [1], and the long-term survival rate for patients with localized Os is about 65%, whereas it is less than 20% for patients with metastatic Os [2]
The results showed that the expression of LncRNA RBM5-AS1 was significantly upregulated in the Os tissues compared with normal tissues (Figure 1(a), P < 0:05)
Os is the most frequent malignant tumor of the bone in the pediatric age group, and the long-term survival rate for patients with localized Os is about 65%, whereas it is less than 20% for patients with metastatic Os

Summary

Introduction

Osteosarcoma (Os) is the most frequent malignant tumor of the bone in the pediatric age group [1], and the long-term survival rate for patients with localized Os is about 65%, whereas it is less than 20% for patients with metastatic Os [2]. The outcome in survival rates highlights the need for novel pathways and targets [3]. Long noncoding RNA (lncRNA) is a large class of RNA molecules with size larger than 200 nucleotides and without protein-coding capability [4]. The functions of lncRNAs range broadly from regulating chromatin structure and gene expression in the nucleus to control messenger RNA (mRNA) processing, mRNA posttranscriptional regulation, cellular signaling, and protein activity in the cytoplasm [5]. Mechanistic investigations showed that RBM5-AS1 activity is critical for the functional enablement of colon cancer stem-like cells [8], but its function in Os is still unclear.

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Conclusion