MicroRNA-99b predicts clinical outcome of osteosarcoma and suppresses tumor cell proliferation, migration and invasion

Xin Shi,Xingfa Guan

doi:10.1186/s13000-019-0889-y

Abstract

BackgroundOsteosarcoma (OS) is a malignancy predominantly occurred in children and adolescents. Numerous microRNAs are involved in the pathogenesis of various cancers. This study aimed to investigate the expression profiles of miR-99b and its prognostic value in OS patients, and further analyze the biological function of miR-99b in the tumor progression by using OS cells.MethodsExpression of miR-99b was measured using quantitative real-time PCR. Kaplan-Meier survival curves and Cox regression analysis were performed to evaluate the prognostic value of miR-99b. OS cell lines were used to investigate the effects of miR-99b on cell proliferation, migration and invasion.ResultsA significant decreased expression of miR-99b was observed in the OS tissues and cell lines respectively compared with the normal tissues and cells. Aberrant expression of miR-99b was associated with the patients’ metastasis and TNM stage, and could be used to predict the prognosis of OS. The expression of miR-99b was regulated in vitro by cell transfection, and we found that the overexpression of miR-99b led to suppressed cell proliferation, migration and invasion, whereas the knockdown of miR-99b resulted in the opposite results.ConclusionsIn one word, the aberrantly expressed miR-99b serves a prognostic biomarker for OS patients. OS cell proliferation, migration and invasion can be inhibited by the overexpression of miR-99b, suggesting that the methods to increase miR-99b expression may be novel therapeutic strategies in OS.

Highlights

Osteosarcoma (OS) is a primary bone tumor and characterized by the bimodal distribution that mainly occurs in children and adolescents [1]
Decreased expression of miR-99b in OS According to the quantitative real-time polymerase chain reaction (qRT-PCR), we found that the expression of miR-99b in the tumor tissues was obviously downregulated compared with the normal tissues (1.34 ± 0.80 vs. 2.91 ± 1.09, P < 0.001, Fig. 1a)
The expression patterns of miR-99b were further confirmed in the OS cells, which performed a significantly lower expression than the normal cells

Summary

Introduction

Osteosarcoma (OS) is a primary bone tumor and characterized by the bimodal distribution that mainly occurs in children and adolescents [1]. Statistics indicate that the incidence of OS is approximately 4.4 per 100,000, and this malignancy ranks the second common primary tumor in bone [2]. Surgical operation is the predominant chose for OS treatment, and the adjuvant therapy, such as chemotherapy, contributes to the reduction of mortality [4]. The prognosis remains dismal for the OS patients diagnosed with advanced tumors [6]. Accurate prognosis and efficient therapy are urgently needed to improve the treatment of OS. Osteosarcoma (OS) is a malignancy predominantly occurred in children and adolescents. This study aimed to investigate the expression profiles of miR-99b and its prognostic value in OS patients, and further analyze the biological function of miR-99b in the tumor progression by using OS cells

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Conclusion