Abstract
Objective To observe the biological activities and expression of extracellular matrix of degenerative nucleus pulposus cells which were cultured in the bone marrow mesenchymal stem cells conditioned medium. Methods Bone marrow mesenchymal stem cells were extracted from the femur and tibia of adult SD rats. After subculture, the conditioned medium of bone marrow mesenchymal stem cells was collected. We destroy the annulus fibrosus of the adult rats, and the intervertebral disc was obtained 2 weeks later. Degenerative rat model was used to extract rat nucleus pulposus cells. The nucleus pulposus cells were cultured in the bone marrow mesenchymal stem cells conditioned medium for 24 hours. Results Compared with normal nucleus pulposus cells, the activity of degenerative nucleus pulposus cells [(59.43±12.33)%, t=11.647, P 0.05) and type Ⅱ collagen in cells (1.52±0.18, t=0.299, P>0.05, 1.21±0.31, t=1.543, P>0.05), proteoglycan (1.81±0.32, t=0.486, P>0.05, 0.99±0.16, t=0.094, P>0.05) and matrix metalloproteinase 3 (0.24±0.14, t=0.486, P>0.05, 0.55±0.11, t=0.593, P>0.05) Protein and mRNA expression levels were not significantly affected. Conclusion The bone marrow mesenchymal stem cell conditioned medium promoted the proliferation of extramedullary nucleus cells and the expression of extracellular matrix proteins. Key words: Intervertebral disc herniation; Nucleus pulposus cells; Extracellular matrix protein; Bone marrow mesenchymal stem cell conditioned medium; Type Ⅱ collagen; Aggrecan; Matrix metalloproteinase 3
Published Version
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