Abstract
ObjectiveMicroRNAs (MiRNAs) is thought to play a critical role in the initiation and progress of ovarian cancer (OC). Although miRNAs has been widely recognized in ovarian cancer, the role of hsa-miR-30a-5p (miR-30a) in OC has not been fully elucidated.MethodsThree mRNA datasets of normal ovarian tissue and OC, GSE18520,GSE14407 and GSE36668, were downloaded from Gene Expression Omnibus (GEO) to find the differentially expressed gene (DEG). Then the target genes of hsa-miR-30a-5p were predicted by miRWALK3.0 and TargetScan. Then, the gene overlap between DEG and the predicted target genes of miR-30a in OC was analyzed by Gene Ontology (GO) enrichment analysis. Protein-protein interaction (PPI) network was conducted by STRING and Cytoscape, and the effect of HUB gene on the outcome of OC was analyzed.ResultsA common pattern of up-regulation of miR-30a in OC was found. A total of 225 DEG, were identified, both OC-related and miR-30a-related. Many DEG are enriched in the interactions of intracellular matrix tissue, ion binding and biological process regulation. Among the 10 major Hub genes analyzed by PPI, five Hub genes were significantly related to the overall poor survival of OC patients, in which the low expression of ESR1,MAPK10, Tp53 and the high expression of YKT,NSF were related to poor prognosis of OC.ConclusionOur results indicate that miR-30a is of significance for the biological progress of OC.
Highlights
Common gynecological malignant tumors have the following categories: ovarian cancer (OC), cervical cancer, endometrial carcinoma, fallopian tube cancer, vulvar cancer and gestational trophoblastic tumor
MiR-30a expression in OC based on Gene Expression Omnibus (GEO) Based on the GEO dataset (Fig. 1), the expression of miR-30a is accessed in a series of OC and normal ovarian tissue (NOT)
While the miR-30 expression of miR-30a in OC tissue increased in the GSE23338 and GSE83693 dataset, and the miR-30a expression of miR-30a in OC tissue decreased in GSE53829 dataset, but all P-values were greater than 0.05
Summary
Common gynecological malignant tumors have the following categories: ovarian cancer (OC), cervical cancer, endometrial carcinoma, fallopian tube cancer, vulvar cancer and gestational trophoblastic tumor. The incidence of OC is lower than that of cervical cancer and endometrial cancer, the lethal rate has far exceeded both, ranking first in. Lu et al Journal of Ovarian Research (2020) 13:120. Rate of young people much higher than that of the elderly. Between 1992 and 2004, the net survival rate increased in Belgium, France, Italy, Portugal, Spain and Switzerland, mainly of young and middle-aged women. The difference in 5-year net survival rates among these countries in 2004 was greater than that in 1992 [4]. It is obvious that the lethal rate of OC is high. If we can find a new target relating to the prognosis of OC, clarify its mechanism, and carry out targeted treatment for OC patients, it can significantly improve the clinical treatment effect and patients’ quality of life, and play an irreplaceable role in monitoring cancer recurrence and guiding rehabilitation treatment
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