Abstract
ObjectiveMicroRNAs (miRNAs) play a vital role in the development of ovarian cancer (OC). The aim of this study to investigate the prognostic value and potential signaling pathways of hsa-miR-9-5p (miR-9) in OC through literature review and bioinformatics methods.MethodsThe expression of miR-9 in OC was assessed using the public datasets from the Gene Expression Omnibus (GEO) database. And a literature review was also performed to investigate the correlation between miR-9 expression and the OC prognosis. Two mRNA datasets (GSE18520 and GSE36668) of OC tissues and normal ovarian tissues (NOTs) were downloaded from GEO to identify the differentially expressed genes (DEGs). The target genes of hsa-miR-9-5p (TG-miR-9-5p) were predicted using miRWALK3.0 and TargetScan. Then the gene overlaps between DEGs in OC and the predicted TG-miR-9-5p were confirmed using a Venn diagram. After that, overlapping genes were subjected to Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Finally, a protein-protein interaction (PPI) network was constructed using STRING and Cytoscape, and the impact of hub genes on OC prognosis was analyzed.ResultsIt was found that OC patients with miR-9 low expression had poor prognosis. A total of 107 DEGs related to both OC and miR-9 were identified. Dozens of DEGs were enriched in developmental process, extracellular matrix structural constituent, cell junction, axon guidance. In the PPI network analysis, 5 of the top 10 hub genes was significantly associated with decreased overall survival of OC patients, namely FBN1 (HR = 1.64, P < 0.05), PRRX1 (HR = 1.76, P < 0.05), SMC2 (HR = 1.22, P < 0.05), SMC4 (HR = 1.31, P < 0.05), and VCAN (HR = 1.48, P < 0.05).ConclusionLow expression of miR-9 indicates poor prognosis of OC patients. MiR-9 plays a crucial role in the biological process of OC by binding to target genes, thus affecting the prognosis of patients.
Highlights
Ovarian cancer (OC) is one of the most common gynecological malignancies, which poses a serious threat to women’s life and health
The results of the random-effects model showed no significant difference in miR-9 expression between the OC tissues and normal ovarian tissues (NOTs) (SMD = 0.03, 95% CI: − 0.99-1.05, P = 0.952)
Based on the comprehensive literature retrieval, it was found that high expression of miR-9 was favorable for OC patients, which could be confirmed by both Overall survival (OS) and progression-free survival (PFS) data
Summary
Ovarian cancer (OC) is one of the most common gynecological malignancies, which poses a serious threat to women’s life and health. About 59% of patients have progressed to an advanced stage when they seek medical treatment due to abdominal distension, ascites, and abdominal pain [3]. The 5-year survival rate of patients with early OC can be greater than 75%, while that of patients with advanced OC is only 29% [2]. With the continuous improvement of diagnosis and treatment, the 5-year survival rate of OC patients has increased from 36% in 1975 to 47% in 2014 [4]. More than 50% of patients with advanced OC relapse within the first 5 years after treatment and develop cross-resistance to standard chemotherapy and other functionally and structurally unrelated chemotherapeutic agents [5]
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