Abstract
The objective of this study was to identify biohydrogenation pathways for linoleic, linolenic, oleic and stearic acids by Orpinomyces species of rumen fungus during in vitro culture. Biohydrogenation of linoleic acid produced conjugated linoleic acid (cis-9, trans-11 C18:2), which was then converted to vaccenic acid (trans-11 C18:1) as the end product of biohydrogenation. Biohydrogenation of linolenic acid produced cis-9, trans-11, cis-15 C18:3 and trans-11, cis-15 C18:2 as intermediates and vaccenic acid as the end product of biohydrogenation. Oleic acid and stearic acid were not converted to any other fatty acid. It is concluded that pathways for biohydrogenation of linoleic and linolenic acids by Orpinomyces are the same as those for group A rumen bacteria.
Highlights
The pathway for rumen bacterial biohydrogenation of linoleic acid (LA) involves three steps
Rate of appearance of the final fatty acid (VA), was significantly affected by added fatty acids (AFA) when all AFA were compared and when LA was compared with linolenic acid (LNA) (AFA.time interaction; p
There was no change in fatty acid proportions in any control culture or blank tubes
Summary
The pathway for rumen bacterial biohydrogenation of linoleic acid (LA) involves three steps. The Final step is hydrogenation of VA to produce stearic acid (SA). This pathway was confirmed by studies involving incubation of unsaturated fatty acids with rumen contents in vivo and in vitro (Noble et al, 1969; Dawson and Kemp, 1970; Choi and Song, 2005; Choi et al, 2006; Wang et al, 2006; Zhang et al, 2006), and by studies with isolated rumen bacteria using pure-culture techniques (Eyssen and Verhulst, 1984; Song and Wang, 2003; Kim et al, 2005; Wang et al, 2005). Biohydrogenation of linolenic acid (LNA) involves four steps; isomerisation, followed by progressive hydrogenation to VA and to SA (Kemp and Lander, 1983; 1984)
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