Biogenic Synthesis of Silver Nanoparticles Using Fruit Extract of Ficus Carica and Study Its Antimicrobial Activity

K Logaranjan,S Devi And K Pandian

doi:10.5101/nbe.v4i4.p177-182

Abstract

Biogenic synthesis of metal nanoparticles is considered to be a cost effective and eco-friendly approach. Here we have demonstrated biogenic synthesis of silver nanoparticles using Ficus Carica extract at room temperature. Ficus Carica fruit extract can be used as reducing as well as capping agent without adding any external reducing agents. The rate of formation of silver nanoparticles was monitored by using UV- Visible spectroscopy by measuring the absorbance at 420 nm for different molar ratios of plant extract to silver nitrate concentrations. The resulting silver nanoparticles was isolated and characterized with various instrumental methods such as FT-IR, XRD and FE-SEM to study the size and shape of particles and functional group of the stabilizing agents. The particles sizes are found to be 20–25 nm ranges with face centered cubic crystal structure of Ag nanoparticles. The antimicrobial activity of silver nanoparticles synthesized by biogenic method was tested against gram negative and gram positive micro-organism. An enhanced antimicrobial activity was observed with silver nanoparticles synthesized from fruit extract when compared with silver nanoparticles synthesized EDTA method.

Highlights

Green synthesis of nanoparticles is an easy, efficient and eco friendly approach, where most researchers are looking at the eco-friendly and green synthesis of nanoparticles paves the way for the researchers around the world to explore the ability to use herbs in synthesizing of metal nanoparticles
The formation of AgNPs during the chemical reaction between the FC extract and AgNO3 is due to reducing agent ability of biologically important molecules such as reducing sugars, flavonoids, saccharides and proteins present in the FC extract which had been discussed in many works
We have demonstrated that the FC extract can be used for the synthesis of AgNPs at room temperature in a green approach

Summary

Introduction

Green synthesis of nanoparticles is an easy, efficient and eco friendly approach, where most researchers are looking at the eco-friendly and green synthesis of nanoparticles paves the way for the researchers around the world to explore the ability to use herbs in synthesizing of metal nanoparticles. Silver nanoparticles (AgNPs) can be synthesized from various parts of the herbal plants like bark of cinnamon zeylanicum [1], azadirachta indica (neem) leaves [2], citrus limon [3], tannic acid [4], parthenium hysterophorus leaf [5], murraya koenigii (curry leaf) [6], lantana camara leaf [7] and various plant leaves [8]. It has been shown that tea leaf and coffee extracts can be utilized to synthesize silver and palladium nanoparticles [9]. Biological organisms like bacteria [10], fungi and actinomycetes [11] were utilized for the synthesis of AgNPs. The synthesis of metal nanoparticles using plant extract (biogenic approach) is still an expanding area of research due to the potential applications in nanomedicines [12]

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Results

Conclusion