An eco-friendly Gnaphalium polycaulon mediated silver nanoparticles: Synthesis, characterization, antimicrobial, wound healing and drug release studies

Abstract

Nowadays, biogenic synthesis of metal nanoparticles using plants has attracted greater in a wide range of pharmaceutical interest owing to posses suitable alternative treatment strategies than existing conventional chemical methods. In the present study, we reported the facile, cost-effective, reproducible biogenic synthesis of silver nanoparticles (Gp-AgNPs) using Indian folk medicinal plant, Gnaphalium polycaulon (Gp) leaves extract. The formation of Gp-AgNPs was confirmed by the assistance of different sophisticated analytical techniques. The synthesized Gp-AgNPs exhibited the maximum SPR peak at 365 nm. FT-IR result indicated the presence of various active functional groups in the extract, which may be responsible for the reduction and stabilization of the Gp-AgNPs. The spherical and triangle shapes with <50 nm size of Gp-AgNPs were authorized by SEM and HR-TEM analyses. The Gp-AgNPs exhibited superior antimicrobial activity against selective Gram-positive (Staphylococcus aureus), Gram-negative (Escherichia coli) bacterial, and fungal (Candida albicans) pathogens. The synthesized Gp-AgNPs suppressed the growth of most dreadful pathogens associated with wound infection. The efficiency of Gp-AgNPs on fastening the rate of healing of a wound was confirmed by in vivo studies. in vitro drug-releasing activity was determined by in vitro dialysis bag method. Concomitantly, the current study is the first attempt to exemplify the potent broad-spectrum of antimicrobial and wound healing efficacies of Gp-AgNPs from G. polycaulon, which could plausibly be a promising biomaterial for various biomedical applications.