Abstract
Methicillin-resistant Staphylococcus aureus is a clinically significant pathogen that causes infections ranging from skin and soft tissue infections to life-threatening sepsis. Biofilm formation by MRSA is one of the crucial virulence factor. Determination of beta-lactamase and biofilm production among Staphylococcus aureus was obtained from various clinical specimens. Standard bacteriological procedures were used for isolation and identification and antibiotic sensitivity was determined using the Kirby Bauer disc diffusion method according to CLSI guidelines. The cloverleaf method, acidometric, iodometric and chromogenic methods were used to detect beta-lactamase while the microtiter plate method and Congo red agar method were used to detect biofilm production. Of the 288 MRSA strains isolated from various clinical specimens,198 (67.07%) were biofilm producers. Cloverleaf and chromogenic (nitrocefin) disc shows 100% results for beta-lactamase detection. Vancomycin was 100% sensitive followed by teicoplanin (92.36%) and linezolid (89.93%). Cloverleaf and nitrocefin disc methods were the most sensitive for detection of beta-lactamase in S. aureus and there was no significant relation between biofilm production and antibiotic sensitivity pattern of S. aureus.
Highlights
IntroductionStaphylococcus aureus) is a dangerous bacteria that can cause minor skin infections to sepsis which can be fatal.[1] The introduction of MRSA has complicated patient management by more extended hospital stays and raising costs while reducing the therapeutic efficacy of current antibacterial drugs.[2] Major clinical crises have ensued from the establishment of resistance.[3] Resistance to Beta-lactam is well-known mechanism of bacterial resistance which can be chromosomally or plasmid-mediated, constitutive or inductive
MRSA (Methicillin-ResistantStaphylococcus aureus) is a dangerous bacteria that can cause minor skin infections to sepsis which can be fatal.[1]
Infected or colonized patients are key reservoirs of MRSA in hospitals and transient hand carriage on the hands of health care personnel is the most common mechanism of patient-to-patient transmission.[17]. In this investigation of 288 methicillin-resistant S. aureus isolates from different clinical specimens majority were isolated from blood 101 (35.07%) followed by pus 97 (33.68 %) and body fluid 2 (0.69 %) whereas Rajaduraipandi et al reported 35.7% of MRSA strains were obtained from throat swabs while 33.6% were collected from pus.[18]
Summary
Staphylococcus aureus) is a dangerous bacteria that can cause minor skin infections to sepsis which can be fatal.[1] The introduction of MRSA has complicated patient management by more extended hospital stays and raising costs while reducing the therapeutic efficacy of current antibacterial drugs.[2] Major clinical crises have ensued from the establishment of resistance.[3] Resistance to Beta-lactam is well-known mechanism of bacterial resistance which can be chromosomally or plasmid-mediated, constitutive or inductive. Infection-causing bacteria and their antibiotic resistance patterns differ dramatically from hospital to hospital.[7] MRSA is the most commonly documented cause of biofilm-associated infections. Because these are commensals on human skin and mucosal surfaces they have a different status. The current investigation was carried out to look for beta-lactamase and biofilm production in S. aureus clinical isolates
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
