Detection of Biofilm Formation in Uropathogenic Bacteria = الكشف عن تكون البيوفيلم في البكتيريا المسببة للأمراض البولية

Abstract

Urinary tract infection (UTI) is the most commonly acquired bacterial infection. Biofilm formation being the prime cause for antibiotic resistance by uropathogenes. The purpose of this study was to detect biofilm formation by uropathogenes isolated from UTIs, their antibiotic susceptibility pattern and detection of genes responsible for biofilm production in some isolates. The isolated bacteria were tested for biofilm production by tube adherence (TA), congo red agar (CRA) and micro titer plate (MtP) methods. Antibiotic susceptibility pattern of uropathogens was done by Kirby-Bauer disc diffusion method and detection of genes responsible for biofilm production in some isolates by PCR. Out of 320 cultures positive cases 146 (46.5 %) isolates were biofilm producers Ecoli was the commonest uropathogen followed by Staphylococcus aureus and Enterococcus feacalis. The antibiotic resistance was higher among biofilm producers to commonly used antibiotics as compared to non biofilm producers. The detection of icaD gene of Staph. areus and cup gene ofpseudomonas by PCR was siginfiacntly related to its detection by congo red agar method, while there no significant relation between detection of ESP gene by PCR and the three used conventional methods. It was concluded that the ability of slime production, adherence and biofilm formation of uropathogenic strains had significant role in antibiotic resistance. Biofilm formation is the major virulence determinant of uropathogen, so it is necessary to screen all urinary isolates for biofilm production.