Abstract
Pesticides are vastly used for pests monitoring in agriculture and public health fields, causing severe depletion in quality of drinking water. Moreover, most insecticides resist biodegradation and carcinogen even at very low levels up to Parts per billion (ppb). This study was carried during April/2019 and extended to January/ 2020 in Directorate of Environment and Water, Baghdad-Iraq to synthesis and characterization silver bionanoparticles by using Bacillus thuringiensis israelensis extract (biological methods) and investigate this activity on the chlorpyrifos pesticide under laboratory conditions. The silver bionanoparticles were characterized using many techniques, X-ray Diffractometer (XRD Fourier Transform Infrared (FTIR), atomic force microscopy (AFM) and zeta potential analyzer spectroscopy. The results of XRD technique confirmed the crystalline nature of the nanoparticles. AFM analysis revealed that particles were spherical, single or in aggregates. Determination the shafting of active groups sites was performed using FTIR. The zeta potential values were -36.33 mV for AgNPs. The biodegradation of chlorpyrifos using silver bionanoparticles was determined quantitatively using high performance liquid chromatography (HPLC) techniques. From the result, it can be suggested that silver bio-nanoparticles from Bacillus thuringiensis israelensis extracts lead to biodegradation of chlorpyrifos completely without forming harmful products confirmed by GC-MS analysis. We endorse that this process has scientific potential in the biodegradation of chlorpyrifos pesticide contaminated water using Green biosynthesis of nanoparticles.
Published Version
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