Bioconversion of the Brown Tunisian Seaweed Halopteris scoparia: Application to Energy

Abstract

The brown Tunisian seaweed Halopteris scoparia was used as a feedstock for producing renewable bioethanol, biogas, and biodiesel to demonstrate the proof of concept for the North African energy sector. A quantitative and qualitative quantification of H. scoparia composition using different colorimetric methods was completed to highlight its bioconversion potential. These substrate inputs were subjected to anaerobic fermentation by Saccharomyces cerevisiae to produce bioethanol. The materials were also used to generate bio-hydrogen and volatile fatty acids during dark fermentation by a bacterial consortium and using the oleaginous yeast Yarrowia lipolytica. The lipids were extracted and trans-esterified to Fatty Acid Methyl Esters (FAMEs), and their profiles were then analyzed with gas chromatography (GC). A significant ratio of the bioethanol, e.g., 0.35 g ethanol/g DW substrate, was produced without pretreatment, consistent with the theoretical Gay-Lussac yield. The production of the biohydrogen and lipids were up to 1.3 mL H2/g DW substrate and 0.04 g/g DW substrate, respectively, from the raw biomass. These results were higher than those reported for other well-studied seaweeds such as L. japonica. Overall, this work contributes to the current investigations in Tunisia for producing alternative energies from algae and finding new solutions to the current energy situation and environmental challenges in Maghreb.