Abstract
Synthesis of 18 S and 28 S ribosomal RNA decreases in KB cells infected with adenovirus type 2 (Ad 2). At late times (18–20 hours) after infection the rate of entry of label into new ribosomes, as determined by analysis of total cellular RNA, polysomes, or free 45 S ribosome subunits is 20% the rate in uninfected cells. The ribosomes made in infected cells enter polysomes and are present in polysomes and free 45 S subunits in the same ratio as newly made ribosomes in uninfected cells. RNA labeled at 19 hours after infection was isolated from the polysome and free 45 S ribosome subunit regions of sucrose gradients and annealed with viral DNA. After a 90-min labeling period, 30% and 10% of the label in the polysome and 45 S subunit fractions hybridizes with Ad 2 DNA. Sucrose gradient analysis and hybridization studies of the RNA labeled 19 hours after infection indicate that ribosomal RNA and viral mRNA continue to enter the ribosome fractions at a constant ratio.
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