Abstract
ABSTRACT Objective Amniotic membrane (AM) extract is a promising tool in regenerative medicine, which offers similar therapeutic effects as intact AM but with a simpler and safer approach. AM displays several subregional variances. Method The quantitative analysis of hepatocyte growth factor (HGF) and transforming growth factor-beta 1 (TGF-β1) in placental and reflected AM sections were assayed by enzyme-linked immunosorbent assay. Results Detection of high levels of HGF and TGF-β1 with variation in concentration between the placental and reflected portions of the same membrane. Conclusion Understanding AM subregional variations would optimize AM extract preparation for specific regenerative medicine therapies.
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