Biochar-bacteria coupling system enhanced the bioremediation of phenol wastewater-based on life cycle assessment and environmental safety analysis

Abstract

The efficient treatment of phenol wastewater is of great necessity since it induces serious pollution of water and soil ecosystems. Using biochar-immobilized functional microorganisms can innovatively and sustainably deal with the existing problem. In this study, we utilized response surface methodology (RSM) combined with life cycle assessment (LCA) to improve phenol biodegradation rate through a novel separated alkali-resistant and thermophilic strain Bacillus halotolerans ACY. Bioinformatic analysis revealed the genetic foundation of ACY to adapt to harsh environments. The characteristics of pig manure biochar (PMB) produced at varying pyrolysis temperatures (300-700 ℃) and adsorption experiment were investigated, immobilization of the phenol-degrading ACY on PMB600 under alkaline and high pollution load promoted phenol removal and extreme environment resistance, and the phenol removal rate reached 99.5% in 7d in actual phenol wastewater, which increased compared with those achieved by PMB (50.6%) and free bacteria (80.5%) alone. Scanning Electron Microscope (SEM) and Fourier transform infrared spectrometry (FTIR) observations indicated the successful bacterial immobilization on PMB600. Reusability and economic cost study further demonstrated PMB600 as an excellent carrier for wastewater treatment. LC-MS, toxicology and carbon footprint analyses demonstrated that bacterial metabolism exerted synergy with adsorption for phenol removal, while biodegradation exerted the predominant impact on the immobilized bacterial system. This study provides an eco-friendly and effective approach to treat phenol wastewater.