Abstract
A simple, sensitive, and rapid UHPLC-MS/MS method was developed for the simultaneous determination of veratraldehyde and its metabolite veratric acid in rat plasma. Cinnamaldehyde was used as an internal standard (IS) and the one-step protein precipitation method with 0.2% formic acid in acetonitrile (mobile phase B) was used for the sample extraction. Reversed C18 column (YMC-Triart C18 column, 50 mm × 2.0 mm, 1.9 µm) was used for chromatographic separation and was maintained at 30 °C. The total run time was 4.5 min and the electrospray ionization in positive mode was used with the transition m/z 167.07 → 139.00 for veratraldehyde, m/z 183.07 → 139.00 for veratric acid, and m/z 133.00 → 55.00 for IS. The developed method exhibited good linearity (r2 ≥ 0.9977), and the lower limits of quantification ranged from 3 to 10 ng/mL for the two analytes. Intra-day precision and accuracy parameters met the criteria (within ±15%) during the validation. The bioanalytical method was applied for the determination of veratraldehyde and veratric acid in rat plasma after oral and percutaneous administration of 300 and 600 mg/kg veratraldehyde. Using the analytical methods established in this study, we can confirm the absorption and metabolism of veratraldehyde in rats for various routes.
Highlights
Veratraldehyde (3,4-dimethoxybenzaldehyde, VD), a derivative of vanillin, is the chemical that is found and isolated from peppermint, ginger, bourbon vanilla, and fruits such as raspberry [1].VD has the vanilla-like and woody savor, so it is widely used as a valuable flavoring and fragrance ingredient [2,3]
Results and Discussion method was developed in this study
There has been no research on the development of assays for the determination of plasma concentrations of VD and its metabolites
Summary
Veratraldehyde (3,4-dimethoxybenzaldehyde, VD), a derivative of vanillin, is the chemical that is found and isolated from peppermint, ginger, bourbon vanilla, and fruits such as raspberry [1]. VD has the vanilla-like and woody savor, so it is widely used as a valuable flavoring and fragrance ingredient [2,3]. In the field of chemistry, the synthesis method of VD has been comprehensively studied and there are many attempts at a catalytic green process, which is an eco-friendly catalytic process, in order to acquire the highest yield [4,5,6]. VD is widely used in the pharmaceutical industry as the intermediate for organic synthesis [7,8,9,10]. Some research showed the repellent effect of VD [13]
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