Bioactivities of Allium longicuspis Regel against anthracnose of mango caused by Colletotrichum gloeosporioides (Penz.)

Dionisio de Guzman Alvindia,Mark Anthony Angeles Mangoba

doi:10.1038/s41598-020-68399-z

Dionisio de Guzman Alvindia, Mark Anthony Angeles Mangoba

Open Access

https://doi.org/10.1038/s41598-020-68399-z

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Abstract

The present study focused on the effect of Allium longicuspis extracts (ALE) against anthracnose of mango fruit. In vitro tests (mycelial growth and conidial germination) showed that, ALE concentrated from 0.75 to 2.5 g L−1 completely inhibited the growth of Colletotrichum gloesporioides. Cytoplasmic discharge, mycelial and conidial blasts were clearly observed when applied with ALE. The minimum effective concentration (MEC) of ALE at 0.75 g L1 can be applied as protective, curative and simultaneous treatment in mango fruit to inhibit the anthracnose infection. Efficacy of garlic extract was relatively superior to synthetic fungicide based on protective, curative and simultaneous treatments. Twenty chemical components were detected in ALE based on GCMS analysis (Table 1). The six major components were the following: oleyl alcohol, methyl ether (42.04%), γ-sitosterol (15.85%), , 24-norursa-3.12-diene (5.62%), 1-octadecanol methyl ether (4.23%), n-pentadecanol (3.95%) and 2-vinyl-4h-1 3-dithiine (3.76%). The findings support the potential use of ALE as an alternative to synthetic fungicide.

Highlights

Mangifera indica L. popularly known as mango, is the second most important agricultural fruit in the Philippines with a production yield of 899.014 metric tons in 20161
Due to continuous used of synthetic fungicides, fungal pathogen developed resistance and it contaminates our e nvironments[10]
This may be useful in managing anthracnose incidence in mango fruit and as an alternative to synthetic fungicides

Summary

Introduction

Mangifera indica L. popularly known as mango, is the second most important agricultural fruit in the Philippines with a production yield of 899.014 metric tons in 20161. Lower concentration (0.75 g L−1) of ALE was needed in vitro to achieve complete inhibition of mycelial growth as compared with the conventional synthetic fungicide (Mancozeb 2.5 g L −1). After 7 days, mango fruit treated with ALE resulted no discoloration nor detectable phytotoxicity was observed even at highest concentration of 2.5 g L −1 (Fig. 5a).

Results

Conclusion