Abstract
1. This is a report of investigations on the nature and the site of binding of streptonigrin to DNA and some of the factors which influence this interaction in vitro and in vivo. 2. There are at least two types of binding of streptonigrin to calf-thymus DNA in vitro: one, which is reversible by dialysis and the other, which is irreversible. The extent of stably bound streptonigrin to DNA was estimated to be 1 mole per 2000 moles deoxynucleotides. 3. More streptonigrin was bound to denatured DNA, apurinic acid and poly dC: poly dG than to native DNA. It was associated preferentially with the dCMP moiety of DNA. Reduction of streptonigrin was not found to be required for binding. 4. Alkaline sucrose gradient centrifugation showed evidence of single strand breaks in streptonigrin-treated DNA, and increased denatured state was indicated by hyperchromicity at 260 nm and increased buoyant density by isopycnic centrifugation in CsCl. 5. With synchronized tissue culture cells, streptonigrin was bound preferentially to DNA during the S (DNA synthesis) period of the cell cycle.
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