Abstract
High-performance liquid chromatographic separations are described for the analysis of hyoscyamine-atropine and scopolamine in combination pharmaceutical dosage forms containing phenobarbital. A mobile phase containing 0.034 M tetramethylammonium phosphate in water–methanol (21:10, pH 2.0) separated hyoscyamine or atropine from scopolamine on an octadecylsilane column in <9 min. Monitoring of the column effluent at 220 nm gave a detection limit of 0.02 μg for each alkaloid. Hyoscyamine sulfate and/or atropine sulfate were determined as total equivalent hyoscyamine sulfate, and scopolamine hydrobromide was determined as a separate entity. Data from the application of the method to commercial pharmaceutical products are also presented.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call