Determination of Some Alkaloids in Vegetative and Rooting Growth of Atropa belladonna L. In Vitro by Using High Performance Liquid Chromatography

Abstract

This study was conducted to determind some alkaloids in Atropa belladonna L. plant by multiplication of nodes cultured on MS medium supplemented different concentreations of BA (0.0, 0.25,0.5 , 0.75, 0.1 ) mg/l, shoots produced in vitro cultured on MS medium supplemented with different concentrations of IBA or IAA (0.0, 0.5, 1.0,1.5, 2.0) for rooting, Then determined some alkaloids in multiple nodes or roots such as Atropine, Hyoscyamine Sulfate, Scopolamine, Tropine. Data refers that highest shoots number 7.00 mg/l shoot/explant achieved from cultered nodes on MS medium supplemented with 0.75 mg/l BA after 8 weeks. Shoots cultured on MS medium supplemented with different concentrations of IBA gave highest roots number 51.30 root/explant from cultered shoots on MS medium supplemented with 1.5 mg/l IBA after 4 weeks. Highest concentrations of Atropine (0.09) mg/g at retention time (3.564) with detected area of (22.6) obtained from roots sample at treatment 1.5 mg/l IBA, Whereas highest concentrations of Hyoscyamine Sulfate (0.10) mg/g at retention time (3.508) with detected area of (24.8) obtained from roots sample at treatment 2.0 mg/l IBA, And this treatment gave highest concentrations of Tropine (0.32) mg/g at retention time (2.914) with detected area of (59.0), highest concentrations of Scopolamine (0.07) mg/g at retention time (3.892) with detected area of (17.7) obtained from roots sample at treatment 0.5 mg/l IBA.