Abstract
AbstractRubber latex wastewater contains high level of inorganic anions such as nitrate and phosphate which are more prevalent as groundwater contaminant. The applicability of biofilm forming bacterial isolates to treat rubber latex wastewater was investigated. Microscopic analyses (CLSM and SEM) evaluated that the major component of biofilm other than bacterial cell is EPS matrix. EPS was found to contain higher proportion of lipids and polysaccharide which are required for attachment and absorption of nutrients. ATR‐FTIR and 1H NMR revealed the functional groups present in EPS. Rhodococcus rhodochrous CTI‐14 showed robust biofilm followed by Cellulosimicrobium sp. CTB‐10. Out of six bacterial consortia tested, consortium‐D efficiently removed 95% –N and 75% . Taguchi method revealed the optimum parameters to remove the nutrients. The consortium‐D optimally reduced nitrate and phosphate at pH 7 at 37°C. Consortium‐D composed of Cellulosimicrobium sp. CTB‐10, Aeromonas veronii OS‐01, Lysinibacillus sphaericus RTA‐01 and Rhodococcus rhodochrous CTI‐14 was found to be the most efficient bacterial consortium to treat rubber latex wastewater.
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