Abstract
The uptake of radioactively labelled DNA into mammalian cells was investigated be means of autoradiography and density gradient centrifugation. The DNA was prepared from Ehrlich ascites tumor cells and Micrococcus lysodeikticus respectively and incubation experiments were run with Ehrlich ascites tumor cells and X 2-cells in tissue culture. DNA uptake is characterized as a function of time, concentration of added DNA, of pH and temperature. Different compounds were tested for interference with DNA uptake. Long-term incubation resulted in a breakdown of added DNA. Most likely, this was due to intracellular DNases demonstrated by discelectrophoresis. Finally, the discussion is dealing with some aspects of the mechanism of DNA uptake into mammalian cells.
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