Abstract
THE presence of polyadenylic acid (poly(A)) tracts in both the heterogeneous nuclear RNA (HnRNA) and polysomal messenger RNA of eukaryotic cells indicates that the high molecular weight HnRNA is the precursor of polysomal mRNA1–3. At least two features of HnRNA, double stranded regions4 and small segments of polyuridylic acid5, seem to be absent from polysomal mRNA. These data and previous observations on the metabolism of HnRNA in vivo6–10 suggest that HnRNA is cleaved and only a selected portion(s) transported to the cytoplasm as functional mRNA.
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