Abstract
Publisher Summary Messenger RNA in animal cells appears to be generated by cleavage of high molecular weight precursors in the nucleus. These mRNA precursors are contained in the metabolically active pool of RNA molecules called heterogeneous nuclear RNA (HnRNA). Polysomal mRNA as well as a substantial part of the HnRNA have been shown to contain 180–200 nucleotide-long segments of poly(A) at the 3' terminus of the polynucleotide chains. In addition to this there is a nonpolysomal pool of ribonuclear protein particles in the cytoplasm that also contains polyadenylated RNA. At present this pool is thought to contain mRNA en route to translation.
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