Abstract

RNA viruses are metastable macromolecular assemblies that sense their environment through dynamic breathing motions. While surfaces of viral particles have been well characterized by cryo-EM, dynamic genome-capsid at the virus’ interior are poorly understood. To address this gap in understanding, we have combined cryo-EM with amide hydrogen deuterium exchange mass spectrometry (HDXMS) on turnip crinkle virus (TCV) as a model system to probe its RNA genome-capsid interface and overall energetics of the virus particle across all stages of programmed disassembly.

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