Abstract
Several reasons may underlie the dramatic increase in type2 diabetes mellitus. One of these reasons is the genetic basis and variations. Vitamin D receptor polymorphisms are associated with different diseases such as rheumatoid arthritis and diabetes. The aim of this study is to investigate the possible association of two identified mutations ApaI (rs7975232) and TaqI (rs731236). Eighty-nine healthy individuals and Fifty-six Type 2 Diabetic (T2D) patients were investigated using RFLP technique for genotyping and haplotyping as well. The distribution of Apal genotypes was not statistically significant among the control (P=0.65) as well as for diabetic patients (P=0.58). For Taql allele frequencies of T allele was 0.61 where of G allele was 0.39. The frequency distribution of Taql genotypes was not statistically significant among the control (P=0.26) as well as diabetic patients (P=0.17). Relative risk of the allele T of Apa1 gene is 1.28 and the odds ratio of the same allele is 1.53, while both estimates were < 1.0 of the allele G. Similarly, with the Taq1 gene the relative risk and the odds ratio values for the allele T are 1.09 and 1.27 respectively and both estimates of the allele C were 0.86 for the relative risk and 0.79 for the odds ratio. The pairwise linkage disequilibrium between the two SNPs Taq1/apa1 was statistically significant in control group (D = 0.218, D' = 0.925 and P value < 0.001) and similar data in diabetic groups (D = 0.2, D' = 0.875 and P value < 0.001). These data suggest that the T allele of both genes Apa1 and Taq1 is associated with the increased risk of type 2 diabetes. We think that we need a larger number of volunteers to reach a more accurate conclusion.
Highlights
The worldwide poor lifestyle is a very concern that underlies a dramatic increase in some health, metabolic disorders, and chronic diseases such as obesity, hypertension, cancers, and diabetes mellitus
There are three famous genetic variation loci of vitamin d receptor ApaI, TaqI and BsmI which are named according to the enzyme that cuts the mutation point at each one
In a study conducted on Taiwanese population scholars have reported that vitamin d receptor polymorphisms were associated with type1 diabetes (Chang et al, 2000)
Summary
The worldwide poor lifestyle is a very concern that underlies a dramatic increase in some health, metabolic disorders, and chronic diseases such as obesity, hypertension, cancers, and diabetes mellitus. There are three famous genetic variation loci of vitamin d receptor ApaI, TaqI and BsmI which are named according to the enzyme that cuts the mutation point at each one. These loci have attracted the scientists to study the possible association between these variants and some diseases among different societies. This study is aimed to investigate the possible association of genetic variation of both vitamin d receptor in ApaI, TaqI loci with the onset of type 2 diabetes among Taif population
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