Association of ST2 gene polymorphism with angiographic severity of coronary artery disease

Abstract

Objective To investigate the relationship between ST2 gene polymorphism and the severity of coronary artery stenosis via angiography. Methods With a case-control design, 352 Hubei Han Chinese patients with coronary heart disease treated at Wuhan Union Hospital from 2008 to 2012 were enrolled in the study. Participants were divided into two groups, the myocardial infarction group and the non-myocardial infarction group, based on clinical data and coronary artery involvement on angiogram. Single Nucleotide Polymor phisms(SNPs) in the ST2 signal transduction pathways chosen from the Hapmap were subjected to analysis using the Sequenom MassArray system and Taqman probes to determine their genotype and allele frequency distribution in myocardial infarction patients and their association with varying degrees of coronary artery involvement in these patients. Results A total of nine SNPs met the inclusion criteria, of which, the frequency distribution of the genotypes TT and CC of the ST2 SNP rs12999364 was higher in the multivessel lesion group than in the single vessel lesion group (P=0.016), while the distribution frequency of the combined genotype TT+ CT was lower in the multivessel lesion group than in the single vessel lesion group (P=0.021). With adjustment for traditional risk factors, including age, gender, smoking, drinking, hypertension, diabetes mellitus, family history of CAD, body mass index, triglyceride and total cholesterol, logistic regression analysis showed that genotypes CT and TT+ CT in rs12999364 were independent protective factors for multivessel lesions not only in CAD (CT: OR=0.471, 95%CI=0.285-0.778, P=0.003; TT+ CT: OR=0.555, 95%CI: 0.347-0.888, P=0.014), but also in the myocardial infarction (MI) subgroup (CT: OR=0.393, 95%CI: 0.189-0.813, P=0.012; TT+ CT: OR=0.453, 95%CI: 0.228-0.901, P=0.024). Conclusions ST2 gene polymorphism rs12999364 is associated with angiographic severity of coronary heart disease and exhibits protective effects for CAD patients and myocardial infarction patients with multivessel lesions. Key words: Coronary artery disease; Gene polymorphism; Coronary arteriography