Association of MnSOD Val16Ala (rs4880) Polymorphism with Staging, Grading, and Molecular Profile of Breast Cancer Patients at Prof. Dr. I.G.N.G. Ngoerah Hospital Denpasar Bali

Abstract

Background: Aging is the major risk factor for breast cancer. Aging and breast cancer are interrelated due to the accumulation of reactive oxygen species (ROS). Manganese superoxide dismutase (MnSOD) has a pivotal role to defense ROS. The MnSOD Val16Ala (rs4880) polymorphism affects the activity of MnSOD in ROS detoxification. This study aims to prove the association of MnSOD Val16Ala (rs4880) polymorphism with stage, grading, and molecular profile of breast cancer patients at Prof. Dr. I.G.N.G. Ngoerah Hospital Denpasar Bali. Methods: This study used a cross-sectional exploratory descriptive method. It was conducted at the Integrated Biomedical Laboratory Unit of the Faculty of Medicine, Udayana University from September to November 2022. The samples used in this study were 45 biological materials in the form of DNA from breast cancer patients who were treated at the Oncology Unit of Prof. Dr. I.G.N.G. Ngoerah Hospital from 2016 to 2018 which is stored in the Biochemistry Laboratory of the Faculty of Medicine, Udayana University. The MnSOD Val16Ala (rs4880) polymorphism was examined by DNA amplification using polymerase chain reaction (PCR) method and the identification of the polymorphism by sequencing. The results obtained were analyzed using descriptive analysis with SPSS program. Results: The sequencing results found 45 samples (100%) with homozygous wild-type T/T. The association between MnSOD Val16Ala (rs4880) polymorphism and staging, grading, and molecular profile was unable to analyze. Conclusions: The MnSOD Val16Ala (rs4880) polymorphism was not found in breast cancer patient in Prof. Dr. I.G.N.G. Ngoerah hospital, Denpasar, Bali. Further research is needed to understand the role of MnSOD Val16Ala (rs4880) polymorphism with breast cancer risk. Keywords: superoxide dismutase enzyme, polymorphism, breast cancer