Association of lipoprotein lipase gene polymorphism and blood triglyceride in patients with type 2 diabetes mellitus

Abstract

Objective To investigate the association between the polymorphisms of the LPL gene and blood triglyceride in type 2 diabetes mellitus(DM). Methods 152 first admission patients with Type 2 DM were chosen from the first affiliated hospital of Zhengzhou University and 145 control subjects were observed. Blood DNA samples were obtained from subjects in both groups, PCR amplification were performed on particular LPL gene fragments, restriction enzyme digestion and their products analyzed.All patients were measured TC, TG, HDL-C, LDL-C, ApoA.ApoB, FBG, HbA1c, HOMA-IR. Detected by statistical methods χ2 test whether the observed genetic equilibrium genotype. T check was used to compare the intergroup blood fat levels, the disparation of genotypic frequency and allele frequency were compared with χ2 test with the test criterion α=0.05. Results LPL gene locus in the case group, Ser447Ter G allele and CG genotypes were lower than that of control group(3.3% vs 12.4%; 6.6% vs 24.8%)and there was statistical significance for all differences(all P<0.05). Both in case group and control group, CG genotype carriers' plasma TG levels were lower than that of non-carrier (respectively: 1.39±0.11 vs 1.68±0.18; 1.24±0.08 vs 1.48±0.15), and the differences have statistical significance (P<0.05). Control groups' CG genotype HDL-C were higher than CC genotype (1.46±0.06 vs 1.35±0.08; P<0.05 ). Asp9Asn and Asn291Ser variants were not found either in T2DM patients or in controls. There were no significant differences in the frequencies of LPL PvuⅡmutations either. And both groups' gene frequencies were precisely in line with the balance law Hardy-Weinberg detection. Conclusions Asp9Asn, Asn291Ser and PvuⅡpolymorphisms in the LPL gene bear no relationship with the type 2 diabetes mellitus in Han people in Henan province. However, the Ser447Ter polymorphism in the LPL gene may reduce plasma triglyceride level, which maybe a protective mutation in reduction of the incidence of type 2 diabetes mellitus. Key words: Diabetes mellitus, type 2; Lipoprotein lipase; Polymorphism, single nucleotide; Triglyceride