Analysis of the association between HLA-DRB(1), DQB(1) gene and pulmonary tuberculosis complicated with diabetes mellitus

Abstract

To analyze the association between HLA DRB(1), DQB(1) allele and pulmonary tuberculosis complicated with type 2 diabetes mellitus among Han nationality of northern Chinese. By using PCR-SSP technique, the genomic DNA typing was applied to compare the difference of the gene frequency between patients and normal controls. The relative risks (RR) of the disease were also estimated. 214 cases were observed, including 123 pulmonary tuberculosis complicated with type 2 diabetes mellitus, 45 type 2 diabetes mellitus, and 46 normal subjects. All these cases were respectively hospitalized in Beijing Thoracic Tumor & Tuberculosis Hospital, Tianjin Lung Disease Hospital, Beijing Thoracic Disease Hospital, Shijiazhuang Diabetes Hospital, from 1998 to 1999. The frequency of DRB(1) * 09 allele in pulmonary tuberculosis complicated with type 2 diabetes mellitus cases was significantly higher than that of DRB(1) * 09 allele in normal controls, 25.10% Vs 14.03%, RR = 2.22, the frequency of DRB(1) * 09 allele in cases was also significantly higher than that of DRB(1) * 09 allele in type 2 diabetes mellitus controls, 25.1% Vs 9.32%, RR = 3.16; the frequency of DQB(1) * 05 allele was significantly lower than that of DQB(1) * 05 allele in normal controls and diabetes mellitus, 7.17% Vs 19.24%, RR = 0.30, 7.17% Vs 21.12%, RR = 0.26. The results indicate that the DRB(1) * 09 allele is susceptive to the pulmonary tuberculosis complicated with type 2 diabetes mellitus, the DQB(1) * 05 may be protective to the pulmonary tuberculosis complicated with diabetes mellitus. The DRB(1) * 09 allele and DQB(1) * 05 allele may affect the incidence of the pulmonary tuberculosis complicated with type 2 diabetes mellitus, or real effect genes link with them.