Association of bovine CXCL8 polymorphism with clinical mastitis and fertility trait in Polish HF cattle

Abstract

Background: The aim of this study was to investigate the relationship between CXCL8 (CXCL8c.105A>G and CXCL8c.210C>T) SNP polymorphism and the clinical mastitis and production trait in Polish HF cattle.Methods: The trait-associated CXCL8 SNPs polymorphism study was carried out in the Polish HF bulls (n=151) by PCR-RFLP methods as described in the previous issue of this journal. The phenotypic records (assessment year 2017) of clinical mastitis, functional and fertility trait were collected from the Research Institute of Animal Production, Balice, Poland (http://www.izookrakow.pl).Results: Overall, the trait-associated study revealed no relationship between the CXCL8c.210C>T SNP polymorphism and the breeding value of selected clinical mastitis and fertility trait of breeding bulls. However, TT genotypes showed low levels of significance of differences for the breeding values of somatic cells count (p = 0.27) and stillborn calving of heifers (p=0.27). Similarly, the trait-associated study showed no correlation between the CXCL8c.105A>G polymorphism and the breeding value of selected clinical mastitis and fertility trait of breeding bulls. However, AA genotypes showed low levels of significance of differences for the somatic cell count (p = 0.12), ease calving of heifers (p = 0.14), the difficulty calving of heifers (p = 0.17), live-born calves of heifers (p = 0.21), and AG genotypes showed the low levels of significance of differences for the stillborn calving in heifers (p = 0.21).Conclusions: Study concludes that trait-associated studies of CXCL8 polymorphism did not identify highly significant effects on clinical mastitis and fertility trait in the investigated breeding bulls of Polish HF cattle.