Association of AGT and ACE gene polymorphism with genetic susceptibility to Henoch-Schonleinpurpura and Henoch-Schonleinpurpura nephritis of adult

Abstract

Objective To explore M235 T polymorphism of angiotensinogen (AGT) gene and I/D polymorphism of angiotensin converting enzyme ( ACE ) gene in renin-angiotensin system,and their relationship with Henoch- Schonlein purpura ( HSP ) / Henoch - Schonleinpurpura nephritis ( HSPN ).Methods Insertion/deletion (I/D ) polymorphism in intron 16 of ACE gene and number two exon M235 T polymorphisms of AGT gene were analyzed in 90 patients with HSP/HSPN and 98 healthy adult controls,by using PCR-RFLP and PCR.Results ①The AGT genotype distribution was significantly different between HSP and normal control,and between HSPN and normal control ( P =0.008,P =0.002 ),but no difference was observed between HSP and HSPN ( P =0.180 ).The AGT gene TT genotype and T allele has a high risk for HSP and HSPN.②The ACE genotype distribution was significantly different between HSPN and normal control ( P =0.003 ),but no difference was observed between HSP and normal control,neither between HSP and HSPN( P =0.065,P =0.073 ).The ACE gene DD genotype and D allele has a high risk for HSPN.Conclusion AGT gene M235T polymorphism is associated with HSP/HSPN.ACE gene I/D polymorphism is associated with HSPN. Key words: Henoch-Schonlein purpura ( HSP ) ;  Henoch-Schonleinpurpura nephritis HSPN ) ;  Angiotensinogen ( AGT ) ;  Angiotensin converting enzyme ( ACE ) ;  Polymorphism