Assessment of the Aqueous Methanol Extract and Ethyl Acetate Fraction of Tapinanthus globiferus Leaves and their Effects on Ulceration and Leukocyte Mobilization in Wistar Albino Rats

Abstract

This study aimed to ascertain the anti-ulcerative, leukocyte mobilizing, haematologic, and antioxidant effect of methanol extract of Tapinanthus globiferus leaves (METGL) and ethyl acetate fraction of Tapinanthus globiferus leaves (EAFTGL) in rat models. Phytochemical analysis of T. globiferus leaves recorded substantial levels of phenolics, flavonoids, alkaloids, saponins, steroids, terpenoids, and tannins. The experimental design was categorized into three phases. Ulcer and inflammation were inflicted on phases I and II rats using indomethacin and inflammatory stimulus (1 mL of 3 % agar suspension), respectively, treatments with reference drug and different concentrations (100 and 200 mg/kg body weight) METGL and EAFTGL ensued. In phase III, group 1 (normal control) received 3 ml/kg bw 3 % tween 80, while groups 2-5 were administered with varied dosages (100 and 200 mg/kg bw) of METGL and EAFTGL, respectively. The results revealed that both METGL and EAFTGL effectively instituted gastro-protection, exerted a significant (p < 0.05) increase in hematological indices and antioxidant parameters, and caused a significant (p<0.05) decrease in leukocyte infiltration. These suggest that T. globiferus leaves may be useful in managing ulcer and inflammatory conditions, validating its use in traditional medicine.